Urinary Excretion of 3-Hydroxyisovaleric Acid Increases and Biotin Decreases during Pregnancy in Mice
نویسندگان
چکیده
The effects of biotin deficiency on maternal metabolism and embryonic development in pregnant mouse dams were studied. In the biotin-deficient group, the inhibition of embryonic development and external malformations such as cleft palate (100 %), micrognathia (100 %) and micromelia (91.4 %) were detected in embryos. Biotin excretion in urine was decreased on day 4 of gestation (dg) and subsequently not detected, and the urinary concentration of 3-hydroxyisovaleric acid (3-HIA) was increased after dg 12. In contrast, the biotin concentration in urine was significantly increased on dgs 4, 8 and 12 in the biotin-supplemented group, and decreased on dg 16 in biotin-supplemented and biotin-control groups. Pyruvic acid in the biotin-deficient group was higher than that in the biotin-supplemented group throughout gestation. These findings demonstrated that the requirement of biotin increases at conception and/or during embryonic development, and a large amount of biotin is necessary for embryonic development in the late stage of gestation.
منابع مشابه
Biotin status: which are valid indicators and how do we know?
Although estimated average requirements for biotin have been proposed, the human requirements for biotin in specific populations and at various ages remain uncertain, in part because indicators of biotin status have not been validated. With the use of improved methods for measuring biotin and metabolites, a recent study indicated that decreased urinary excretion of biotin and bisnorbiotin is an...
متن کاملMarginal biotin deficiency is common in normal human pregnancy and is highly teratogenic in mice.
In studies of marginal biotin deficiency induced experimentally in adults, increased urinary excretion of 3-hydroxyisovaleric acid (3HIA), which likely reflects decreased activity of the biotin-dependent enzyme beta-methylcrotonyl-CoA carboxylase, and decreased activity of the biotin-dependent enzyme propionyl-CoA carboxylase (PCC) in peripheral blood lymphocytes have been validated as indices ...
متن کاملSmoking accelerates biotin catabolism in women.
BACKGROUND Smoking accelerates the degradation of many nutrients, including lipids, antioxidants, and certain B vitamins. Accelerated biotin catabolism is of concern in women because marginal biotin deficiency is teratogenic in mammals. OBJECTIVE The objective was to assess the effect of smoking on the biotin status of women. DESIGN A preliminary study of 7 women and 3 men examined the urin...
متن کاملAdequate intake of biotin in pregnancy: why bother?
Frank, symptomatic biotin deficiency is a rare occurrence. The only well-documented cases have occurred in association with total or near total intravenous feeding without biotin supplementation, during chronic consumption of un-denatured egg white, and with inborn errors of metabolism that lead to biotin wasting (1, 2). A single case that does not fit any of these established associations is t...
متن کاملMeasurement of 3-hydroxyisovaleric acid in urine of biotin-deficient infants and mice by HPLC.
We developed an assay for measuring urinary 3-hydroxyisovaleric acid (3-HIA) using HPLC after derivatization with 2-nitrophenylhydrazine hydrochloride (2-NPH . HCl). The derivatized 3-HIA was extracted into n-hexane and separated isocratically on a C8 reversed-phase column for fatty acids (YMC-Pack FA). We used this method to measure 3-HIA in urine extracts from mice fed a biotin-deficient diet...
متن کامل